Rockland Rabbit anti Soybean Trypsin Inhibitor antibody was used to detect purified Soybean Trypsin Inhibitor. Samples of ~1 and 0.25 ug of purified Soybean Trypsin Inhibitor per lane were run by SDS-PAGE under reducing and non-reducing conditions and reduced samples of protein contained 4% BME and were boiled for 5 minutes. Protein was transferred to nitrocellulose and probed with Rockland Rabbit anti Soybean Trypsin Inhibitor antibody (200-4679 lot 6594 1:5K in MB-0070, ON 4 C). Primary antibody was detected with Rockland Dylight 488 conjugated Streptavidin (S000-41 Lot 21091, 1:5K) and Atto 425 conjugated goat anti rabbit (611-151-122 lot 26423C 1:10K 1.5 hr RT in MB-070) and imaged on the BioRad VersaDoc imaging system.

Western Blot of Rabbit Anti-Trypsin Inhibitor Antibody. Lane 1: Opal PreStained Molecular Weight Marker (p/n MB-210-0500). Lane 2: Trypsin Inhibitor [50ng]. Primary Antibody: Anti-Trypsin Inhibitor at 1µg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) 1:40,000 for 30mins at RT. Block: BlockOut Buffer (p/n MB-073) for 30mins at RT. Expect: ~25kDa.

Rockland Biotin conjugated Rabbit anti-trypsin inhibitor antibody (200-4679 lot 6594) and Rabbit anti streptavidin (200-4195 lot 23495) were used to detect target proteins Trypsin Inhibitor (left) and Streptavidin (right) under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1µg of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:1000 dilution of primary antibody (ON 4°C). Detection shown was using DyLight 649 conjugated Donkey anti rabbit (611-743-127 lot 20831 1:10K 1.5 hr RT in MB-070) and imaged on the BioRad VersaDoc System